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Nakhwa Arkhaesi

ABSTRACT

Background: Vitamin A has central role in immune system. Several studies suggest an interaction between vitamin A and malaria. IL-12 is a cytokine that have potency to induce cellular immune defense, and also inhibit and cytotoxic to intracellular parasites such as malaria. The objectives of this study was to investigate correlation between vitamin A and IL-12 on asymptomatic children in endemic malaria area

Methods: A cross sectional study was conducted on 2 villages with highest malaria HCI in  Purworejo District, Central Java, Indonesia. Seventy seven children age 6-14 years  were randomly selected as study subjects. Children with fever (>37.2°C) or symptomatic malaria were excluded. Malaria diagnosis was confirmed by Rapid diagnostic test (RDT), thick and thin peripheral blood smears. Vitamin A and IL-12 were measured from vein blood samples by ELISA methods.

Results: From 77 blood samples, RDT yield 5 children were negative, 70 children were infected by P. falciparum and 2 children by P. vivax. however by blood smear examination only 2 samples were positive for P. vivax. The level of vitamin A was  28.97 ± 8.009 μg/L. and serum IL-12 was 3.36 ±

3.327 pg/mL Spearman’s rho correlation analysis shows significant correlation between vitamin A and IL-12 (r=0.32;p=0.005).

Conclusion: Blood vitamin A is correlated to serum IL-12 on asymptomatic malaria children.

Keywords: asymptomatic, malaria, vitamin A, IL-12

ABSTRAK

 

Latar belakang: Vitamin A memiliki peran sentral dalam kekebalan tubuh. Beberapa penelitian menunjukkan adanya interaksi antara kadar vitamin A dengan malaria. IL-12 merupakan sitokin yang dapat menginduksi respon imun. IL-12 dapat  menghambat dan efek sitotoksik terhadap parasit intrasel termasuk malaria. Penelitian ini bertujuan untuk mengetahui hubungan antara kadar Vitamin A dan IL-12 serum pada anak dengan malaria asimtomatih di daerah endemis malaria.

Metode: Penelitian belah lintang dilakukan di 2 desa dengan HCI malaria tertinggi di Kabupaten Purworejo, Jawa Tengah. Subyek penelitian adalah 77 anak usia 6–14 tahun yang dipilih secara acak. Anak dengan demam (>37,2°C) dan ada gejala malaria tidak digunakan sebagai sebyek penelitian. Diagnosis malaria dilakukan dengan Rapid diagnostic test (RDT), preparat hapus darah tepi tipis dan tebal. Kadar vitamin A dan IL-12 diukur dari sampel darah vena dengan metode ELISA.

Hasil: Pemeriksaan RDT menunjukkan 5 anak negative, 70 anak terinfeksi P, falciparum dan 2 anak dengan P. vivax. Kadar vitamin A serum 28,97 ± 8,009 μg/L dan kadar IL-12 serum 3,36 ± 3,327 pg/mL. Uji korelasi Spearman menunjukkan korelasi positif yang bermakna antara kadar vitamin dan IL-12 serum ((r=0,32;p=0,005).

Simpulan: Kadar vitamin A berkorelasi dengan IL-12 serum pada anak dengan malaria asimtomatik di daerah endemis malaria

Kata kunci: malaria asimtomatik, vitamin A, IL-12.

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